Intro

The Functional Genomics Platform at Whitehead Institute provides advice and reagents to researchers interested in implementing large-scale CRISPR/Cas9 screens in mammalian cells.

We use the CRISPR/Cas9 system to identify modulators of different cellular phenotypes, such as viability and the output of reporter systems.

The FGP is run by Heather Keys, Ph.D.

David Sabatini, M.D., Ph.D. is the faculty advisor for the FGP.

On this website, you can find more details about our work, our team, contact information, and protocols.

Work

We assist researchers at all stages of the screening process, including planning, implementation and validation of genetic screens:

  • Experimental design
  • sgRNA library design and propagation
  • Virus preparation for optimization and screening
  • Vector design
  • Sequencing library preparation
  • Data analysis
  • Follow-up strategies

  • Publications

    • *Li Y, *Muffat J, *Javed AO, *Keys HR, Lungjangwa T, Bosch I, Khan M, Virgilio MC, Gehrke L, Sabatini DM, Jaenisch R (2019) Genome-wide CRISPR screen for Zika virus resistance in human neural cells. PNAS . 2019 Apr 24. doi: 10.1073/pnas.1900867116
      *equal contribution
    • Tsvetkov P, Detappe A, Cai K, Keys HR, Brune Z, Ying W, Thiru P, Reidy M, Kugener G, Rossen J, Kocak M, Kory N, Tsherniak A, Santagata S, Whitesell L, Ghobrial IM, Markley JL, Lindquist S, Golub T (2019) Mitochondrial metabolism promotes adaptation to proteotoxic stress. Nature Chemical Biology: Manuscript accepted.
    • Kory N, Wyant GA, Prakash G, Uit de Bos J, Bottanelli F, Pacold ME, Chan SH, Lweis CA, Wang T, Keys HR, Guo YE, Sabatini DM (2018) SFXN1 is a mitochondrial serine transporter required for one-carbon metabolism. Science 362(6416). 2018 Nov 16. doi: 10.1126/science.aat9528
    • Kanarek N, Keys HR, Cantor JR, Lewis CA, Chan SH, Kunchok T, Abu-Remaileh M, Freinkman E, Schweitzer LD, Sabatini DM (2018) Histidine catabolism is a major determinant of methotrexate sensitivity. Nature 559:632-636. 2018 July 11. doi: 10.1038/s41586-018-0316-7

    Heather Keys, Ph.D.

    Research Scientist and Manager, Functional Genomics Platform

    FGP manager


      Mehreen Khan

      Technical Assistant I


      Olena Fedoniuk

      Technical Assistant I


      Former and current Masters Students

      Manuel Mastel, Heidelberg University

      Nicole Eischer, IMC University of Applied Sciences Krems

Materials

Here you can find basic protocols for screening and related experiments. More coming!

Any questions? Contact us

Protocols

  • Titer Test
  • Genomic DNA Maxiprep
  • Bead Cleanup and dsDNA Quantification

  • Coming Soon!: Transduction Test for New Cell Lines
  • Coming Soon!: Large-scale Lentiviral Preparation
  • Coming Soon!: Small-scale Lentiviral Preparation
  • Coming Soon!: Genomic DNA Miniprep
  • Coming Soon!: Sequencing Library Preparation
  • Coming Soon!: Transduction Conditions for Cancer Cell Lines
  • Coming Soon!: Materials Reference List

  • Join Us

    We are looking for motivated people to work with us.

    If you are a motivated Masters student searching for an exciting internship experience, contact us!

    Contact Us

    Please contact Heather Keys, Ph.D. by email with all questions.

    We are located at the Whitehead Institute, 455 Main Street, Cambridge, MA.

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